Fig. 2

Transcriptional control of γc expression via TCR signaling. A Upregulation of surface γc expression by IL-2. CD8⁺ T cells were stimulated with γc cytokines (10 ng/ml IL-2, 10 ng/ml IL-7, and 100 ng/ml IL-15) or non-γc cytokine (10 ng/ml IFN-γ) for 16 h, and γc and IL-7Rα expression was analyzed using FACS. Data are representative of three independent experiments. B Upregulated expression of surface γc partially blocked by 10 µg/ml α-IL-2. CD8⁺ T cells were stimulated with α-TCR/α-CD28 in the absence or presence of α‐IL‐2. Surface expression of IL-7Rα and γc was determined using FACS. The results are the summary of three independent experiments. C Removal of transcriptional regulation completely suppresses upregulation of γc expression. CD8⁺ T cells from B6, γcTg, and γcKOγcTg mice were assessed for γc surface (left), IL-7Rα (middle), and CD69 (right). γc, IL-7Rα, and CD69 expression (open histogram) versus control antibody staining (shaded histogram) are shown for CD8⁺ T cells from B6, γcTg, and γcKOγcTg mice. The results are the summary of three independent experiments. D CD8⁺ T cells were stimulated with α-TCR/α-CD28 in the presence or absence of Bay11 and INCA6 as specific inhibitors for NFκB and NFAT1, respectively. γc (left), CD69 (middle), and TCRβ (right) surface expression was analyzed using FACS. Histograms show the representative data from four independent experiments. The bar graph shows the relative surface expression of γc, CD69, and TCRβ. The relative γc, CD69, and TCRβ expression was calculated as the Δ mean fluorescence intensity (ΔMFI) associated with the inhibitor-treated group over the ΔMFI associated with the DMSO control group. DMSO was used as vehicle control. Data are presented as the mean ± standard error of the mean of three independent experiments. ** p < 0.01