Fig. 5

NFAT1 and NFκB cooperatively activate the γc promoter. A Schematic of the mutated NFκB-binding sites -440 (GG to TT), -180 (GGG to TTA), and -114 (GG to TT and TC to TT) regions on the γc promoter. B HEK293 T cells were transfected with control pGL4-luc or each of the pGL4-NFAT1-luc plasmids that carried point mutation at the -440, -180, and -114 sites of the γc promoter region and were stimulated with PMA/Iono for 6 hr. The results are the summary of three independent experiments. C Schematic of mutated NFAT1-binding sites of the -737 (GA to TC) and -111 (TC to GA) region on the γc promoter. D HEK293 T cells were transfected with control pGL4-luc or each of the pGL4-NFκB-luc plasmids that carried point mutations at the -737 and -111 sites of the γc promoter region and were then stimulated with PMA/Iono for 6 hr. The results are the summary of three independent experiments. E HEK293 T cells were transfected with the indicated plasmids that carried the entire γc promoter region and were then stimulated with PMA/Iono for 6 hr. Luciferase activity in the lysates of transfected cells was determined by a dual luciferase assay. Data are presented as the mean ± standard error of the mean of four independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001