Fig. 7

γc overexpression inhibits IL-7 signaling. A and B Surface γc expression in WT and γcTg CD4⁺ and CD8⁺ T cells (open histogram) as overlaid with control antibody (shaded histogram) staining: (A) the γc expression level was determined by flow cytometry, and (B) summary of the γc expression level data. C and D IL-7 downstream signaling in γc-overexpressing CD8⁺ and CD4⁺ T cells: (C) IL-7-stimulated LN T cells from WT and γcTg mice were assessed for pSTAT5 expression using flow cytometry, and (D) bar graphs show fold induction of IL-7-induced STAT5 phosphorylation over IL-7-untreated cells. Data are representative of eight independent experiments. E LN T cell numbers determined in IL-7Tg and IL-7TgγcTg mice. Each symbol represents an individual mouse. Horizontal lines indicate the mean ± standard error of the mean. F Spleen weight of IL-7Tg and IL-7TgγcTg mice. Each symbol represents an individual mouse. G Gross anatomy of spleens and LNs from the indicated mice. Image shows representative spleen and lymph nodes from > 5 mice per group. NS, non-significant; * p < 0.05, ** p < 0.01, and *** p < 0.001