Fig. 3

Characterization of EVs obtained with precipitation method. A Western blot for tetraspanins (CD81, CD9), TSG101 and RPS6 was performed on EVs purified with precipitation method and derived from MDA-MB-453 and MCF-7 cells treated with DMSO and Sorafenib. Lysate from SK-Hep1 cell was used as a positive control. B Transmission electron microscopy (TEM) precipitated EVs showed vesicles with characteristic morphology and size of EVs. Scale bar, 100Â nm. C Nanoparticle tracking analysis showed heterogeneous size-distribution profiles of the EVs derived from DMSO or sorafenib treated breast cancer cells