Full-length and N-terminally truncated recombinant interleukin-38 variants are similarly inefficient in antagonizing interleukin-36 and interleukin-1 receptors

Table 1 Molecular weights and concentrations of recombinant proteins

recombinant protein	MW (kDa)	concentration	equivalent concentration
recombinant protein	MW (kDa)	(ng/ml)	(nM)
aa2-152His IL-38	18	10	0.6
		100	5.6
		1000	55.6
aa2-152 IL-38	17	10	0.6
		100	5.9
		1000	58.8
aa3-152 IL-38	17	10	0.6
		100	5.9
		1000	58.8
aa20-152 IL-38:Fc-KIH	78	30	0.4
		300	3.8
		3000	38.5
IL-36Ra	17	10	0.6
		100	5.9
		1000	58.8
sIL-1Ra	17	1	0.06
		10	0.6
		100	5.9
		1000	58.8
icIL-1Ra1	18	1	0.06
		10	0.6
		100	5.6
IL-36g	17	0.1	0.006
IL-36g	17	10	0.6
IL-1a	18	1	0.06
Fc-KIH IgG1	28	17	0.6
		170	6.1
		1700	60.7

The molecular weights (MW) of the recombinant proteins included in this study, and their concentrations used in cell-based assays, expressed in ng/ml and nmol/L (nM), are indicated

ISSN: 1478-811X