Fig. 3

Sos1 depletion alters focal adhesion dynamics. (A) Representative IRM images of individual primary MEFs from the four indicated genotypes, taken from timelapse microscopy movies. Boxes indicate regions shown in enlarged images in (B). Scale bar in B: 10 μm. (C) Individual MEFs in A are represented with a color code (red, green and blue) representing the stability of FA throughout the duration of the timelapse experiments (60 min) scaling from low (blue), medium (green) and high (red) stability, respectively. (D) Cell spread area of MEFs from the four genotypes was evaluated by IRM. Images were generated by serially overlapping all movie images (1 image every 20 s during 60 min). (E) Images illustrate the cell perimeter of individual MEFs at different time points using a color code that represents the relative cell position at the beginning of the experiment (time 0, red) at 30 min (green) and at 60 min (blue), respectively. In addition, white color represents convergence zones and illustrates highly stable FA. Scale bar: 25 μm. (F-I) Bar charts represent the number of FA per cell (F) the relative percentage of intensity of FA (G), cell spread area (H) and the number of lamellipodia per cell in MEFs of the four genotypes. n = 12 cells per genotype (from three experimental replicates) (F and I), n = 9 per genotype (from three experimental replicates) (G) and n = 10 per genotype (from three experimental replicates) (H). Data are expressed as mean ± SEM. Statistical differences were considered at: */# p < 0.05, **/## p < 0.01 and ***/### p < 0.001 vs. Sos1/2^WT and Sos2^KO, respectively