Fig. 4

Sos1 depletion alters cellular Rac-GTP localization. Representative confocal microscopy images of primary MEFs of the four defined experimental groups stained with for actin filaments with phalloidin (green), with GST-PBD followed by anti-GST antibodies to detect GTP-loaded Rac (red) and with DAPI to stain nuclei. Dotted lines indicate the site of the scratch. Arrows indicate accumulation of Rac-GTP near to the scratched sites at 30 min after the scratch. Scale bar: 10 μm. The plot shows the mean intensity of Rac-GTP immunoflorescent signal in MEFs of the four defined groups, through the cytoplasm and the free edges, of all experimental groups. The intensity was quantified by measuring the signal intensity using five 10 μm-long straight lines per cells perpendicularly to the wound). n = 3 independent experiments per experimental group (15 cells per condition)