Fig. 6

Sos1/2- depletion increased Mmp2/9 expression. (A). Quantification of mRNA expression levels of Mmp2 and Mmp9 in primary MEFs of the indicated Sos genotypes by RT-qPCR. β-actin was used as internal control for normalization. n = 3 independent samples per genotype. Data expressed as mean ± SD. *p < 0.05 and **p < 0.01 vs. Sos1/2^DKO; #p < 0.05 vs. Sos1/2^WT. (B) Representative western blots of Sos1, Sos2, phospho-Myosin, phospho-Fak, total Myosin, total Fak, and Mmp2 protein expression, using Tubulin as a control for normalization. Bar charts illustrate the quantitation of the ratio of phospho-myosin levels relative to total myosin and the ratio of Mmp2 protein expression relative to Tubulin expression in MEFs of all Sos1/2 genotypes. n = 3 per genotype. Data shown as mean ± SD. *p < 0.05 and **p < 0.01 vs. Sos1/2^DKO. && p < 0.01 vs. Sos1^KO and Sos1/2^DKO