Fig. 7

Mmp2/9 inhibitors block Sos1/2-dependent gelatin degradation. (A) 12 days 4OHT-treated MEFs of the four defined genotypes were seeded on gelatin-FITC-coated coverslips for 8 h and then fixed. The graph illustrates the mean area of gelatin degradation (black areas). Data shown as mean ± SD. n = 3 independent experiments per genotype (a total of 50 cells were quantitated per experiment). ****p < 0.0001 vs. Sos1/2^WT, #p < 0.05 vs. Sos1^KO. (B-C) Primary MEFs treated as in A were plated on gelatin-FITC-coated coverslips and pre-treated with ARP100 (B) or Ilomastat (C) as indicated in the Material and Methods section, 3 h after seeding. The cells were then fixed after 16 h. The graph illustrates the mean area of gelatin degradation (black areas). Data shown as mean ± SD. n.s: not significant. n = 5 independent experiments per genotype (a total of 50 cells were quantitated per experiment)