Fig. 11

Effect of SS on chemoresistance of OC cells to BOLD-100. (A) Viability of cells treated with BOLD-100 in concentration range 10–500 µM for 48 h measured by crystal violet assay. Results expressed as percentage of solvent control. (B) Viability of cells treated with BOLD-100 in concentration range 10–100 µM for 48 h for SKOV3 and 50–200 µM for 48 h for OVCAR3 with or without preconditioning with 24 h SS measured by crystal violet assay. Results expressed as percentage of solvent control for both static and SS conditions. * depicts significant difference between static and SS conditions. (C) Viability of cells exposed to 24 h SS with or without presence of LOVA (1 µM) and afterwards treated with BOLD-100 in concentration 50 µM (SKOV3), 100 µM (OVCAR3) for 48 h, measured by crystal violet assay. * depicts significant difference between solvent control and treatments, # depicts significant difference between BOLD-100 treatment and combination LOVA and BOLD-100. (D) Viability of cells exposed to 3 h SS with or without presence of GsMTx4 (1 µM) and afterwards treated with BOLD-100 in concentration 50 µM (SKOV3), 100 µM (OVCAR3) for 48 h, measured by crystal violet assay. * depicts significant difference between solvent control and treatments, # depicts significant difference between BOLD-100 treatment and combination GsMTx4 and BOLD-100. Statistical significance was calculated with t-test and multiple comparisons by one-way ANOVA test and shown as */# p < 0.05, **/## p < 0.01, ***/### p < 0.001