Fig. 3
Shear stress stimulation in EOC: cell morphometric adjustment. (A) Quantification of actin cytoskeleton of cells exposed to SS (3 h and 24 h) and respective static controls. Actin intensity is expressed as MFI [r.f.u.], cytoplasmic area is expressed in [µm2]. In representative images, actin is depicted in green, nuclei in blue. (B) Quantification of the Young’s modulus median per ROI [kPa] for cytoplasmic areas of the SKOV3 and OVCAR3 cells exposed to SS (3 h and 24 h) and respective static controls. Representative AFM maps show the height depicted in pink, the Young’s modulus representing maps depict values between 0–20 kPa (shown in blue-to-red). (C) Quantification of PM intensity [r.f.u.] in cells exposed to 24 h SS and in respective static controls. In representative images, PM depicted in magenta, nuclei in blue. Statistical significance calculated with t-test and shown as * p < 0.05, ** p < 0.01, *** p < 0.001