Fig. 5

Shear stress stimulation in EOC: impact on cholesterol biosynthesis and uptake (A) Quantification of cholesterol level [r.f.u.] in cells exposed to 1 h, 3 h and 24 h of SS and in respective static control. Representative images show cholesterol at plasma membrane depicted in blue. (B) Quantification of cholesterol level [r.f.u.] in cells exposed to 1 h, 3 h and 24 h of SS in presence of LOVA (1 µM) for 1, 3–24 h, respectively. * depicts significant differences between LOVA treatment and solvent control (C) Quantification of LDLR expression [r.f.u.] at the plasma membrane in cells exposed to 3 h and 24 h of SS and in respective static controls. In illustrative images LDLR depicted in red. (D) Quantification of cholesterol level [r.f.u.] in cells exposed to 3 h SS and in respective static controls in presence of 10% serum (control) or 0% serum. * depicts significant differences between 10% serum (control) and 0% serum. # depicts difference between static and SS. Representative images show cholesterol at plasma membrane depicted in blue. All experiments were performed in at least 3 independent biological replicates. Statistical significance calculated with t-test and shown as */# p < 0.05, **/## p < 0.01, ***/### p < 0.001