Fig. 6

Shear stress stimulation in EOC: mechanosensitive regulation of YAP1. A + B Quantification of YAP1 n/c ratio and nuclear signal in cells exposed to 1 h, 3 h and 24 h of SS expressed as percentage of static control. Representative images show YAP1 depicted in red. Heatmap represents kinetics of changes in nuclear (NUC), cytoplasmic (CYTO), total YAP1 protein level and n/c ratio. Data expressed as percentage of static control. * depicts significant differences between SS and static control, # depicts significant differences between SS 1 h and SS 3 h, SS 24 h, respectively. C + D Quantification of LATS1 and p-LATS1 in nuclear and cytoplasmic compartment of the cells exposed to 1 h, 3 h SS, expressed as percentage of static control. Representative images show LATS1 and p-LATS1 depicted in red. All experiments were performed in at least 3 independent biological replicates. Statistical significance calculated with t-test and shown as */# p < 0.05, **/## p < 0.01, ***/### p < 0.001