Fig. 4
From: AlkTango reveals a role for Jeb/Alk signaling in the Drosophila heart
Jeb overexpression affects later heart function. A Column graph depicting developmental lethality (in %) of 4xHand-Gal4 control (mean = 2.612 +/- 2.594 SD), 4xHand>UAS-jeb (mean = 9.166 +/- 6.396 SD), and 4xHand>UAS-Alk.EC (mean = 4.569 +/- 4.201 SD). One way Analysis of Variance (ANOVA) reveals no significant (ns) difference between the genotypes. B Kaplan Meier curve of 4xHand-Gal4 control, 4xHand>UAS-jeb and 4xHand>UAS-Alk.EC flies cultured on standard diet at 29 °C. C Column graph depicting lethality (in %) 4xHand-Gal4 control (mean = 5 +/- 7.07 SD), 4xHand>UAS-jeb (mean = 56.8 +/- 11.1 SD), and 4xHand>UAS-Alk.EC (5 +/- 7.07 SD). Kruskal-Wallis test was used to reveal statistical significance (*** = P < 0.001, ns = non-significant). C´ Bee swarm box and whiskers plot depicting climbing performance after exposure to 32°C at the indicated time points. Single dots represent average values from three measurements from groups of ten flies (n = 60 flies for each genotype). One way Analysis of Variance (ANOVA) was used to test for statistical significance (*** = P < 0.001, ** = P 0.009, * = P 0.048, ns = non-significant). D-F Anti-GFP (green), anti-Alk or anti-Jeb (red) antibody staining of dorsal vessels from third instar 4xHand>GMA control (D), 4xHand>GMA+Jeb (E) and 4xHand>GMA+Alk.EC (F) 3rd instar larvae. DAPI (blue) labels nuclei. Arrows highlight the ostial myofibrillar content. G-I Anti-GFP (green) and anti-RFP (red) antibody staining of dorsal vessels from third instar 4xHand>RedStinger control (G), 4xHand>RedStinger+Jeb (H) and 4xHand>RedStinger+Alk.EC (I) larvae in the Zasp66::GFP background. Arrows highlight the ostial myofibrillar content. J-L Anti-GFP (green) and anti-RFP (red) antibody staining of dorsal vessels from 4xHand>RedStinger control (J), 4xHand>RedStinger+Jeb (K) and 4xHand>RedStinger+Alk.EC (L) 3rd instar larvae in the background of the toll305-GFP reporter. DAPI (blue) labels nuclei. Asterisks mark the position of the cardiac valve. M Quantification of pupal heart rates for control 4xHand-Gal4>UAS-RedStinger/+; UAS-EYFP/+, 4xHand-Gal4>UAS-RedStinger/UAS-jeb; UAS-EYFP/+ and 4xHand-Gal4>UAS-RedStinger/+; UAS-EYFP/UAS-Alk.EC pupae (n = 12 animals for each genotype). Students t-test was applied to test for statistical significance (*** = P < 0.001, ns = non-significant)