Table 1 Primer sets used in the present study
From: Glucose- and glutamine-driven de novo nucleotide synthesis facilitates WSSV replication in shrimp
Gene | Primer | Primer sequence (5’-3’) | Function |
|---|---|---|---|
G6PDH (PVHP204597.4) | G6PDH-qF | 5’-CGCCACCATCAACAACGA-3’ | Real-time PCR |
G6PDH-qR | 5’-AGGGCCTTTCCGCATCTG-3’ | Real-time PCR | |
G6PDH-dsF | 5’-AAGCTGTCGAACCACCTG-3’ | Cloning | |
TKT (PVHP71465.2) | TKT-qF | 5’-CACTGCCTTCCAGCATGACA-3’ | Real-time PCR |
TKT-qR | 5’-ATTCCAGCCAAAACCTTCGA-3’ | Real-time PCR | |
TKT-dsR | 5’-GAGACAAAAGCAACAGTACGGT-3’ | Cloning | |
TKT-qF-T7 | 5’-TAATACGACTCACTATAGGGAGACACTGCCTTCCAGCATGACA-3’ | dsRNA synthesis | |
TKT-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGAGAGACAAAAGCAACAGTACGGT-3’ | dsRNA synthesis | |
PRPS (PVHP205382.2) | PRPS-qF | 5’-TCTATCGCGAGCCCCAATT-3’ | Real-time PCR |
PRPS-qR | 5’-GGCCACGGAGAGCATGTT-3’ | Real-time PCR | |
PRPS-dsR | 5’-AGACTCCTTCATGTGCTG-3’ | Cloning | |
PRPS-qF-T7 | 5’-TAATACGACTCACTATAGGGAGATCTATCGCGAGCCCCAATT-3’ | dsRNA synthesis | |
PRPS-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGAAGACTCCTTCATGTGCTG-3’ | dsRNA synthesis | |
ATase (PVHP244493.1, PVHP244501.1) | Atase-qF | 5’-TCCGTTGATGCTGATATTATTTCC-3’ | Real-time PCR |
Atase-qR | 5’-ATCCCATAGCAGCCGGAGTA-3’ | Real-time PCR | |
ADSS (PVHP165389.1) | ADSS-qF | 5’-CGGATGCGTCCCCTTGT-3’ | Real-time PCR |
ADSS-qR | 5’-GTTGCTATGGAGACACTTGTGGAT-3’ | Real-time PCR | |
IMPDH (PVHP227689.1) | IMPDH-qF | 5’-AAGCACAGGAGGTCATGAAAGTC-3’ | Real-time PCR |
IMPDH-qR | 5’-GGCAAACAGGGTCACGAATG-3’ | Real-time PCR | |
IMPDH-dsR | 5’-TCAACACCAGCATTGACAAG-3’ | Cloning | |
IMPDH-qF-T7 | 5’-TAATACGACTCACTATAGGGAGAAAGCACAGGAGGTCATGAAAGTC-3’ | dsRNA synthesis | |
IMPDH-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGATCAACACCAGCATTGACAAG-3’ | dsRNA synthesis | |
CAD (PVHP274591.1) | CAD-qF | 5’-CGGTTCAAGTTTTCGCGTATG-3’ | Real-time PCR |
CAD-qR-F2-dsR | 5’-TGGGCTGACTTGAGGTTGGT-3’ | Real-time PCR | |
CAD-F1-dsF | 5’-CATTGGTCCTCCGTCAAT-3’ | Cloning | |
CAD-F1-dsR | 5’-CCGTGATTCTGAGACGTG-3’ | Cloning | |
CAD-F2-dsF | 5’-AGAAGCACAACATCATGCCT-3’ | Cloning | |
CAD-F3-dsF | 5’-AACATGTCCTGAGTGTTC-3’ | Cloning | |
CAD-F3-dsR | 5’-GCATACTGTGTGACATAG-3’ | Cloning | |
CAD-F1-dsF-T7 | 5’-TAATACGACTCACTATAGGGAGACATTGGTCCTCCGTCAAT-3’ | dsRNA synthesis | |
CAD-F1-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGACCGTGATTCTGAGACGTG-3’ | dsRNA synthesis | |
CAD-F2-dsF-T7 | 5’-TAATACGACTCACTATAGGGAGAAGAAGCACAACATCATGCCT-3’ | dsRNA synthesis | |
CAD-F2-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGATGGGCTGACTTGAGGTTGGT-3’ | dsRNA synthesis | |
CAD-F3-dsF-T7 | 5’-TAATACGACTCACTATAGGGAGAAACATGTCCTGAGTGTTC-3’ | dsRNA synthesis | |
CAD-F3-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGAGCATACTGTGTGACATAG-3’ | dsRNA synthesis | |
DHODH (PVHP148100.1) | DHODH-qF | 5’-GGCAGCAGGATTTGATAAGCAT-3’ | Real-time PCR |
DHODH-qR | 5’-AAAGCTGAATCCCATACGAAACA-3’ | Real-time PCR | |
DHODH-dsR | 5’-AGAAACGGTTGTGTTGCTT-3’ | Cloning | |
DHODH-qF-T7 | 5’-TAATACGACTCACTATAGGGAGAGGCAGCAGGATTTGATAAGCAT-3’ | dsRNA synthesis | |
DHODH-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGAAGAAACGGTTGTGTTGCTT-3’ | dsRNA synthesis | |
UMPS (PVHP164355.1) | UMPS-qF-dsF | 5’-AACTCATGTGGGCCAAAC-3’ | Real-time PCR |
UMPS-qR | 5’-GGCTACCCCGCAAACCAG-3’ | Real-time PCR | |
UMPS-dsR | 5’-TAACTCCGCAGAGGTGGT-3’ | Cloning | |
UMPS-dsF-T7 | 5’-TAATACGACTCACTATAGGGAGAAACTCATGTGGGCCAAAC-3’ | dsRNA synthesis | |
UMPS-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGATAACTCCGCAGAGGTGGT-3’ | dsRNA synthesis | |
CMPK (PVHP117215.1) | CMPK-qF | 5’-CCTTGTTAGAACGTGCCATGAA-3’ | Real-time PCR |
CMPK-qR | 5’-CCGAGGAAATCCATCAATGAG-3’ | Real-time PCR | |
CMPK-dsF | 5’-CCAAGCTTATGTTTTTGAATCGAATT-3’ | Cloning | |
CMPK-dsR | 5’-CCGGATCCACTGAACAGCTTTTCAAC-3’ | Cloning | |
CMPK-dsF-T7 | 5’-TAATACGACTCACTATAGGGAGAATGTTTTTGAATCGAATT-3’ | dsRNA synthesis | |
CMPK-dsR-T7 | 5’-TAATACGACTCACTATAGGGAGAACTGAACAGCTTTTCAAC-3’ | dsRNA synthesis | |
Luciferase | Luc-F | 5’-CTGAATACAAATCACAGAATC-3’ | Cloning |
Luc-R | 5’-GCGAGAATCTGACGCAGGCAGT-3’ | Cloning | |
Luc-F-T7 | 5’-TAATACGACTCACTATAGGGAGACTGAATACAAATCACAGAATC-3’ | dsRNA synthesis | |
Luc-R-T7 | 5’-TAATACGACTCACTATAGGGAGAGCGAGAATCTGACGCAGGCAGT-3’ | dsRNA synthesis | |
EF-1α | EF-1α-qF | 5’-ACGTGTCCGTGAAGGATCTGAA-3’ | Real-time PCR |
EF-1α-qR | 5’-TCCTTGGCAGGGTCGTTCTT-3’ | Real-time PCR | |
WSSV IE1 | IE1-qF | 5’-CCAGGCCCAGTGTCATACG-3’ | Real-time PCR |
IE1-qR | 5’-AGAAATCTCATCACATGTCAAATCAGA-3’ | Real-time PCR | |
WSSV VP28 | VP28-qF | 5’-AGTTGGCACCTTTGTGTGTGGTA-3’ | Real-time PCR |
VP28-qR | 5’-TTTCCACCGGCGGTAGCT-3’ | Real-time PCR | |
Others | Anchor-dTV | 5’-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV-3’ | Reverse transcription |