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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Dual inhibition of GTP-bound KRAS and mTOR in lung adenocarcinoma and squamous cell carcinoma harboring KRAS G12C

Fig. 2

Clonogenic assay in three cell lines treated using three KRAS G12C inhibitors and mTOR inhibitor. (A) Representative image of the clonogenic assay in H23 cell lines treated with AMG 510 and RAD001. The colony growth is suppressed in a dose-dependent manner by monotherapy and stronger inhibited by combination therapy. The heatmap represents the amount of colony growth by the density of crystal violet. (B, C) Two representative images of the clonogenic assay in HOP-62 (B) and Calu-1 (C) cell lines after treatment with AMG 510 and RAD001. In both cell lines, the colony growth is suppressed in a dose-dependent manner and stronger in combination therapy. (D) Summary of the clonogenic assay in cell lines treated with three different KRAS G12C inhibitors and RAD001. Cell growth is suppressed in a dose-dependent manner. Combination index is below 0.8 (dashed line) in all three cell lines in three different agents. (E) Results of the clonogenic assay in H520, which is lung squamous cell carcinoma cell lines without KRAS mutation. Neither colony growth inhibition in a dose-dependent manner nor higher suppression by combination treatment is observed. Combination index is above 0.8 (dashed line) in all concentrations, being non-synergistic in H520 cell lines

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Cell Communication and Signaling

ISSN: 1478-811X