Fig. 2
From: Cytochrome P450 2E1 aggravates DXR-induced myocardial injury through imbalanced mitochondrial OPA1
Establishment of myocardium-specific Cyp2e1 overexpression and knockout rats. (A) Experimental strategy for generating myocardium-specific CYP2E1 tool rats. (B-C) generating of conditional Cyp2e1 knockout (Cyp2e1 cKO) rats using the Cre-loxP recombination system. Crossbreeding with α-MHC-Cre rats produced myocardium-specific CYP2E1 knockout rats (Cyp2e1-KO; Cyp2e1flox/flox/α-MHC-Cre). LA: left arm; RA: right arm. (D-E) Immunoblotting of CYP2E1 in cardiac tissues from Cyp2e1-KO rats to assess knockout efficiency. Quantitative analysis normalized to GAPDH. (F) Strategy for constructing myocardium-specific CYP2E1 overexpression rats (Cyp2e1-OV using a-MHC (a-myosin heavy chain)-driven transgene expression. (G) Generation of Cyp2e1-OV rats through crossing with SD wild-type rats. (H-J) Validation of CYP2E1 overexpression efficiency in total and mitochondrial protein extracts from Cyp2e1-OV cardiac tissues via immunoblotting. Quantitative analysis was normalized to GAPDH or VDAC1. Data shown are mean ± SEM; n = 9 per group. Two-group comparisons were executed using unpaired two-tailed Student’s t-tests. **P < 0.01, ***P < 0.001