Fig. 5
From: Cytochrome P450 2E1 aggravates DXR-induced myocardial injury through imbalanced mitochondrial OPA1
CYP2E1 expression affects myocardial injury, mitochondrial metabolism, and myocardial apoptosis under DXR stimulation. (A) Experimental strategy for transcriptomic analysis and protein interaction mass spectrometry using myocardium-specific CYP2E1 animal models and stable H9c2 cell lines with high expression of Flag-CYP2E1. (B) Transcriptomic analysis of myocardium from Cyp2e1-KO or Cyp2e1-OV rats. Volcano plot shows up-regulated (pink) and down-regulated (green) genes. The x-axis represents log2fold-change, indicating the direction of gene expression changes (negative for downregulation, and positive for upregulation). The y-axis illustrates the negative log of the FDR-adjusted p-value, indicating the significance of changes. (C) Heatmap of differentially expressed genes involved in cardiomyopathy, mitochondrial metabolism, and apoptosis. (D) Enrichment analysis of RNA sequencing data, highlighting enriched pathways. Bubble color indicates significance, while bubble size corresponds to the number of DEGs in each pathway. (E) Enrichment analysis of CYP2E1-bound proteins identified by mass spectrometry following CYP2E1 immunoprecipitation. Key gene ontology terms and KEGG pathways are displayed and categorized into biological process, cellular component, molecular function, and KEGG pathway.