Table 1 Comparison summary of five extracellular vesicles extraction methods
Isolation method | UC | exoEasy | TEI | EVtrap | ÄKTA |
|---|---|---|---|---|---|
EVs internalization |  + +  + +  |  + +  + +  +  |  +  |  +  |  + +  +  |
Total identified proteins | 1929 | 1709 | 1677 | 2773 | 1704 |
The ratio of identified proteins in Vesiclepedia | 91% | 91% | 91% | 90% | 91% |
The ratio of identified proteins in Exocarta | 81% | 82% | 77% | 68% | 82% |
Total identified N-glycans | 54 | 50 | 45 | 60 | 46 |
Total isolation time (h) | 2.5 | 0.5 | 14 | 2 | 0.25 |
Hands on time (min) | 20 | 10 | 10 | 30 | — |
Specialized equipment | Yes | No | No | No | Yes |
Easy to perform | *** | * | * | ** | ***** |
Cost | $ | $$$$ | $$$ | $$$$$ | $ |