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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Induced mitochondrial deficit by NDUFS3 transient silencing reduces RAB7 expression and causes lysosomal dysfunction in pancreatic cancer cells

Fig. 3

Differences in mitochondrial morphology in NDUFS3-silenced YAPC and MIA PaCa-2 cells compared to controls. (A) Representative electron micrographs of YAPC and MIA PaCa-2 cells transfected with siRNA#1 and siRNA#2, respectively, or with control RNA (Scr). Scale bars: 1 μm. (B-C) Evaluation of the cristae number and (D-E) of the short mitochondrial axis width in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2, respectively) compared to their Scr control. (F) Mitochondria were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using MitoTracker Green FM dye. White boxes indicate zoomed areas below. Scale bar: 10 μm. (G-H) Corrected total cell fluorescence (CTCF) of mitochondria was determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001

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Cell Communication and Signaling

ISSN: 1478-811X