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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: The ester derivative Palmitoylcarnitine abrogates cervical cancer cell survival by enhancing lipotoxicity and mitochondrial dysfunction

Fig. 1

Ectopic expression of DOC2B in SiHa alters the global metabolite profile of EVs. A Representative Western blot image and bar graph confirming the ectopic expression of DOC2B in SiHa cells. B The EV size distribution was measured by nanoparticle tracking analysis (NTA). C Size distribution of EVs collected from Vector transfected (Control) and DOC2B transfected (DOC2B) SiHa cells. D Western blotting analysis of EVs collected from Vector transfected and DOC2B transfected SiHa cells and probed for CD63, CD9, and DOC2B. E The Venn diagram represents common and unique metabolites identified by LC–MS analysis of EVs. F Principal component analysis (PCA) performed using EV metabolites from Vector transfected and DOC2B transfected SiHa cells. G The metabolite distribution in EVs collected from Vector transfected and DOC2B transfected SiHa cells. H The metabolite Palmitoylcarnitine (PC) abundance in control and DOC2B EVs. In DOC2B EVs, the PC level is significantly increased compared to control EVs. I Cytotoxic effects of PC on various cell lines. The bar graph represents the percentage cell viability of SiHa, HeLa, Fibroblast, HaCaT, Cal27, and MCF7 cells in response to PC exposure for 48 h as analyzed by MTT assay. Data presented are mean ± SD of three independent experiments in triplicate (Mito C-Mitomycin C (10 µg/ml)). *P < 0.05, **P < 0.01, and ***P < 0.001 indicates statistical significance

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Cell Communication and Signaling

ISSN: 1478-811X