Fig. 2
Modulation of TNF-α mRNA and protein expression by exogenous PGE2. Primary Kupffer cells (KC), peritoneal macrophages (PM) and bone marrow-derived macrophages (BMDM) were stimulated for 24 h with LPS and/or PGE2. (A) Relative mRNA expression of TNF-α was determined by RT-qPCR with Hprt as the reference gene. (B) TNF-α protein levels were quantified by ELISA in cell culture supernatants. Values are median (line), upper- and lower quartile (box) and extremes (whiskers) of (A) n = 33 (KC), n = 26 (PM) or n = 29 (BMDM) or (B) n = 27 (KC), n = 26 (PM) or n = 26 (BMDM) independent experiments. Statistics: Two-way-ANOVA with Tukey´s post hoc test for multiple comparison. *p < 0.05