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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Dynamic changes in macrophage populations and resulting alterations in Prostaglandin E2 sensitivity in mice with diet-induced MASH

Fig. 5

Modulation of LPS-mediated expression of cyclooxygenase 2 (COX-2) and secretion of prostaglandin E2 (PGE2) in macrophages from wildtype controls (WT) and COX-2-deficient mice (KO). Primary Kupffer cells (KC), peritoneal macrophages (PM) and bone marrow-derived macrophages (BMDM) were stimulated for 24 h with LPS. (A) Relative mRNA expression of COX-2 (gene name Ptges2) was determined by RT-qPCR with Hprt as the reference gene. (B) Protein lysates were analyzed by immunoblotting for COX-2 protein expression, with β-Actin serving as the loading control. All original blots are provided in Supplementary figure S5-7. (C) PGE2 level were determined by ELISA in cell culture supernatants. Values are median (line), upper- and lower quartile (box) and extremes (whiskers) of n = 26–32 (WT) or n = 5–10 (KO) independent experiments. Statistics: Two-way-ANOVA with Tukey´s post hoc test for multiple comparison. *p < 0.05. n.d.: not detectable

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Cell Communication and Signaling

ISSN: 1478-811X