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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Prkci promotes colorectal cancer metastasis by phosphorylating and stabilizing Tgfbr1 to activate TGF-β signaling

Fig. 4

Prkci promoted metastatic signaling by activating the TGF-β pathway. (A) Gene Set Enrichment Analysis (GSEA) of single-cell RNA sequencing data revealed enrichment of TGF-β signaling in metastatic colorectal cancer samples compared to primary tumors. (B, C) Western blot analysis showed the expression level of target proteins in the designated cell lines. (D) MG132 and CQ were applied to treat the designated cell lines. Western blot showed the expression level of target proteins. (E, F) His-ubiquitin plasmids were transfected into the designated cell lines. MG132 was used to the degradation of endogenous Tgfbr1. Immunoprecipitation and Western blotting assays showed the ploy-ubiquitination level of Tgfbr1. (G) SW48 cells were transfected with K11R His-ubiquitin plasmids. IP and IB analysis to show the poly-ubiquitination of Tgfbr1 in KO-NC or KO-Prkci cells. (H) IP and IB analysis to show the K11-polyubiquitination of Tgfbr1 in KO-NC or KO-Prkci cells using anti-ubiquitin K11 linkage antibody. Each western blot assay was performed in triplicate, yielding consistent results. Each western blot assay was performed in triplicate, yielding consistent results

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Cell Communication and Signaling

ISSN: 1478-811X