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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Epigenetic modulation of the NLRP6 inflammasome sensor as a therapeutic modality to reduce necroptosis-driven gastrointestinal mucosal dysfunction in HIV/SIV infection

Fig. 6

Chronic THC administered in combination with ART significantly reduced HIV/SIV infection induced p-MLKL(Ser358) and HMGB1 protein expression in the intestine. p-MLKL(Ser358) (green) (A-C), and HMGB1 (green) (I-K) protein expression in colon tissues of uninfected control (A&I), VEH/SIV (B&J), and THC/SIV (C&K) RMs. p-MLKL(Ser358) (green) (E-G) and HMGB1 (green) (M-O) expression in jejunum tissues before SIV infection (E&M) and at 5 months post SIV infection in ART suppressed VEH/SIV (F&N) and THC/SIV (G&O) RMs. Representative immunofluorescence images were captured using a Zeiss confocal microscope at 20X magnification. Quantitation of p-MLKL(Ser358) (D&H), and HMGB1 (L&P) average positive area fluorescence in colon and jejunum epithelium was performed using the HALO software. Note the significantly reduced p-MLKL(Ser358) and HMGB1 staining in the jejunum epithelium of ART suppressed THC/SIV (G, O, H&P) compared to VEH/SIV (F, N, H&P) RMs. White arrows in panels A to G indicate epithelial regions. Immunofluorescence data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison post hoc test. A p-value of < 0.05 was considered significant. Controls refer to uninfected controls

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Cell Communication and Signaling

ISSN: 1478-811X